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KMID : 0644120120250010001
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Journal of Oriental Gynecology
2012 Volume.25 No. 1 p.1 ~ p.10
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Inhibitory Effects of Achyranthis Radix Extract Mixed with Hydrogel on Osteoclast Differentiation
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Choi Jin-Young
Lee Chang-Hoon
Jang Jun-Bock
Lee Kyung-Sub
Lee Jin-Moo
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Abstract
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Purpose: This study was conducted to evaluate the inhibitory effect of Achyranthis Radix extract(ARE) loaded hydrogel on osteoclast differentiation.
Methods: MTT-assay was performed to estimate cytotoxicity of ARE, Achyranthis Radix-alginate hydrogel disk(ARHD) in bone marrow macrophages stimulated(BMMs) with human receptor activator of nuclear factor- ?B ligand(RANKL), human macrophage-colony stimulating factor(M-CSF). Tartrate resistant acid phosphatase staining and RT-PCR were performed to know the inhibitory effect on osteoclast differentiation. Reactive oxygen species and actin ring formation were analysed to observe the effect of ARHD.
Results: ARE has no cytotoxicity at the concentration of 0.1 mg/mL or lower. ARE decreased the number of TRAP positive cells in RANKL, M-CSF stimulated BMMs and the gene expression. ARHD has no cytotoxicity at the concentration of 10㎍/mL (24, 48hours), 50㎍/mL (24 hours). ARHD restrained the synthesis of reactive oxygen species and the formation of actin ring.
Conclusions: Achyranthis Radix has the inhibitory effect of osteoclast differentiation and bone resorption. Further studies are needed to treat osteoporosis by Achyranthis Radix.
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KEYWORD
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Achyranthis Radix, Osteoclast differentiation, Hydrogel, RANKL
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